{
  "slug": "utilization-of-flow-cytometry-metabolomic-analyses-and-a-felin-40422255",
  "source": {
    "title": "Utilization of Flow Cytometry, Metabolomic Analyses and a Feline Infectious Peritonitis Case Study to Evaluate the Physiological Impact of Polyprenyl Immunostimulant.",
    "journal": "Cells",
    "year": 2025,
    "doi": "10.3390/cells14100752",
    "pmid": "40422255",
    "content_access_status": "FULL_TEXT_OA"
  },
  "claims": [
    {
      "statement": "The metabolomic analyses identified sphinganine and ceramide as common metabolites upregulated in association with PI exposure in THP-1 cells and mice.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "99",
      "evidence_excerpt": "In this metabolomic study, we identified that sphinganine and ceramide were the common metabolites upregulated by PI treatment.",
      "review_status": "PASSED"
    },
    {
      "statement": "PI exposure did not significantly alter bone marrow or splenic hematopoietic stem and progenitor cell populations in the mouse experiments.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "99",
      "evidence_excerpt": "Figure 6 B shows the flow cytometry results of the bone marrow versus the splenic HSPCs, represented by the LSK cells; PI does not significantly impact the cell populations.",
      "review_status": "PASSED"
    },
    {
      "statement": "The authors identify the single concentration and single time point used for metabolomics as a limitation requiring further experiments.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "100",
      "evidence_excerpt": "But the metabolomic experiments performed in this study were monitored at a specific concentration of PI at only one time. As such, additional metabolomic experiments conducted at different PI treatment times and concentrations will be needed to provide a more robust analysis, which will provide more support for the participation of PI in sphingolipid metabolism, and reveal the molecular targets.",
      "review_status": "PASSED"
    },
    {
      "statement": "Mouse bone marrow immunophenotyping showed lower percentages of B cells and CD8+ cells and increased CD4+ cells after PI exposure.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "99",
      "evidence_excerpt": "Figure 6 C shows that PI decreases the percentage of B cells and CD8+ cells in BM, but increases CD4+ cells.",
      "review_status": "PASSED"
    }
  ],
  "provenance": {
    "agent": "AVAPROJECT-IMMUNOLOGY-002",
    "critic": "AVAPROJECT-CRITIC-001",
    "model": "openai-codex",
    "updated": "2026-10-31 11:30 UTC",
    "updated_iso": "2026-10-31T11:30:30Z"
  }
}