{
  "slug": "molecular-detection-of-feline-viruses-in-free-ranging-european-42768131",
  "source": {
    "title": "Molecular detection of feline viruses in free-ranging European wildcats (Felis s. silvestris) of the Harz Mountains, Germany.",
    "journal": "Archives of virology",
    "year": 2026,
    "doi": "10.1007/s00705-026-06744-9",
    "pmid": "42768131",
    "content_access_status": "FULL_TEXT_OA"
  },
  "claims": [
    {
      "statement": "The authors identify prolonged storage in ethanol at − 20 °C as a potential limitation on recovery of amplifiable nucleic acids.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "C",
      "confidence": "100",
      "evidence_excerpt": "This storage method is not optimal for long-term nucleic acid preservation, as prolonged storage in ethanol at − 20 °C may reduce the recovery of amplifiable nucleic acids over time [ 90 – 92 ].",
      "review_status": "PASSED"
    },
    {
      "statement": "The authors interpret FeLV proviral DNA detection as indicating previous or ongoing infection, without determining active replication or infection outcome.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "C",
      "confidence": "100",
      "evidence_excerpt": "Detection of FeLV proviral DNA indicates previous or ongoing infection but does not allow conclusions regarding active viral replication or infection outcome.",
      "review_status": "PASSED"
    },
    {
      "statement": "The survey examined 42 road-killed European wildcats recovered in the Harz Mountains between 2010 and 2018.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "C",
      "confidence": "100",
      "evidence_excerpt": "Between 2010 and 2018, 42 road-killed wildcats in the Harz Mountains in central Germany (Fig. 1 ) were recovered and stored at − 20 °C until necropsy.",
      "review_status": "PASSED"
    },
    {
      "statement": "FeLV was molecularly detected in 5 of 42 samples, with a reported detection frequency of 11.9% and 95% confidence interval of 4.0–25.4.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "C",
      "confidence": "100",
      "evidence_excerpt": "Feline leukemia virus was detected in 5 of 42 (11.9%; 95% CI 4.0–25.4) samples.",
      "review_status": "PASSED"
    },
    {
      "statement": "The source and direction of FeLV transmission involving the sampled wildcats remain unresolved.",
      "epistemic_type": "OPEN_QUESTION",
      "evidence_level": null,
      "confidence": "100",
      "evidence_excerpt": null,
      "review_status": "PASSED"
    }
  ],
  "provenance": {
    "agent": "AVAPROJECT-VIROLOGY-001",
    "critic": "AVAPROJECT-CRITIC-001",
    "model": "openai-codex",
    "updated": "2026-10-31 11:30 UTC",
    "updated_iso": "2026-10-31T11:30:30Z"
  }
}