{
  "slug": "long-terminal-repeats-of-gammaretroviruses-retain-stable-expre-39459853",
  "source": {
    "title": "Long Terminal Repeats of Gammaretroviruses Retain Stable Expression after Integration Retargeting.",
    "journal": "Viruses",
    "year": 2024,
    "doi": "10.3390/v16101518",
    "pmid": "39459853",
    "content_access_status": "FULL_TEXT_OA"
  },
  "claims": [
    {
      "statement": "In the K562 reporter-vector experiments, the FeLV-derived vector displayed expression stability comparable to MoMLV.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "99",
      "evidence_excerpt": "The FeLV-derived vector displayed expression stability comparable to the stability observed with MoMLV.",
      "review_status": "PASSED"
    },
    {
      "statement": "Most retargeted active proviruses remained in predicted chromatin A compartments, limiting conclusions about expression in strongly repressive chromatin.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "99",
      "evidence_excerpt": "On the other hand, although retargeted proviruses are more distant from promoters and enhancers, most of the retargeted active proviruses are still located within predicted chromatin A compartments and thus can be supported by the transcriptionally permissive chromatin environment ( Figure 2 ).",
      "review_status": "PASSED"
    },
    {
      "statement": "The authors observed a uniform silent-population proportion across wild-type and BET-independent vectors bearing FeLV LTRs, unlike the increased silent population reported for retargeted MLV LTR vectors.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "99",
      "evidence_excerpt": "The phenomenon of an increased proportion of the silenced population after integration retargeting seems to be specific to MLV LTR as we observed a uniform proportion of the silent population among wt and Bin vectors bearing FeLV and SNV LTRs.",
      "review_status": "PASSED"
    },
    {
      "statement": "In the ASLV-derived self-inactivating vector comparison, AS.FeLV and AS.SFFV had estimated active proviral fractions between 80 and 90% at 14 days post infection.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "99",
      "evidence_excerpt": "We then estimated the active genomes ratio and observed that AS.SFFV together with AS.FeLV are the most active vectors, producing between 80 and 90% of active proviruses at 14 dpi.",
      "review_status": "PASSED"
    },
    {
      "statement": "The mechanisms of early post-integration silencing of gammaretroviral LTRs in somatic cells remain an open research question.",
      "epistemic_type": "OPEN_QUESTION",
      "evidence_level": null,
      "confidence": "99",
      "evidence_excerpt": null,
      "review_status": "PASSED"
    }
  ],
  "provenance": {
    "agent": "AVAPROJECT-VIROLOGY-001",
    "critic": "AVAPROJECT-CRITIC-001",
    "model": "openai-codex",
    "updated": "2026-10-31 11:30 UTC",
    "updated_iso": "2026-10-31T11:30:30Z"
  }
}