{
  "slug": "integrase-anchors-viral-rna-to-the-hiv-1-capsid-interior-41708858",
  "source": {
    "title": "Integrase anchors viral RNA to the HIV-1 capsid interior.",
    "journal": "Nature",
    "year": 2026,
    "doi": "10.1038/s41586-026-10154-x",
    "pmid": "41708858",
    "content_access_status": "FULL_TEXT_OA"
  },
  "claims": [
    {
      "statement": "HIV-1 integrase promotes encapsulation of viral genomic RNA into mature viral cores.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "98",
      "evidence_excerpt": "HIV-1 integrase (IN) promotes encapsulation of viral genomic RNA into mature viral cores, and this function is a target for ongoing antiretroviral drug development efforts 1-3 .",
      "review_status": "PASSED"
    },
    {
      "statement": "The authors determined a cryo-EM structure of primate lentiviral integrase in complex with RNA that formed a linear filament of integrase octamer repeat units.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "98",
      "evidence_excerpt": "Here we determined the cryogenic electron microscopy (cryo-EM) structure of a primate lentiviral IN in a complex with RNA, revealing a linear filament made of IN octamer repeat units, each comprising a pair of asymmetric homotetramers.",
      "review_status": "PASSED"
    },
    {
      "statement": "In the SIVtal integrase-RNA TAR cryo-EM structure, integrase tetramers engage RNA chains solely through their C-terminal domains.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "97",
      "evidence_excerpt": "The tetramers engage RNA chains solely through their CTDs.",
      "review_status": "PASSED"
    },
    {
      "statement": "HIV-1 integrase filament repeat spacing matches the mature HIV-1 capsid hexamer lattice spacing of 91 Å.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "96",
      "evidence_excerpt": "The spacing between unit cells in the mature CA lattice (hexamers) and in the IN filament (octamers) is identical - 91 Å, as indicated by arrows.",
      "review_status": "PASSED"
    },
    {
      "statement": "Substitutions in residues involved in HIV-1 integrase-capsid contacts produced eccentric virions with RNA nucleoids outside the cores.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "98",
      "evidence_excerpt": "Substitutions of residues involved in IN-CA contacts yielded eccentric virions with RNA nucleoids located outside of the cores.",
      "review_status": "PASSED"
    },
    {
      "statement": "The paper does not provide direct evidence about FeLV or cats.",
      "epistemic_type": "OPEN_QUESTION",
      "evidence_level": null,
      "confidence": "95",
      "evidence_excerpt": null,
      "review_status": "PASSED"
    }
  ],
  "provenance": {
    "agent": "AVAPROJECT-VIROLOGY-001",
    "critic": "AVAPROJECT-CRITIC-001",
    "model": "openai-codex",
    "updated": "2026-09-05 17:24 UTC",
    "updated_iso": "2026-09-05T17:24:54Z"
  }
}