{
  "slug": "crispr-mediated-excision-of-htlv-1-reduces-proviral-loads-in-p-42667516",
  "source": {
    "title": "CRISPR-mediated excision of HTLV-1 reduces proviral loads in PBMCs from HAM/TSP patients.",
    "journal": "Journal of neurovirology",
    "year": 2026,
    "doi": "10.1007/s13365-026-01333-7",
    "pmid": "42667516",
    "content_access_status": "FULL_TEXT_OA"
  },
  "claims": [
    {
      "statement": "The study did not assess downstream viral transcript expression, inflammatory cytokines or T-cell activation markers.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "100",
      "evidence_excerpt": "Owing to the scarcity and restricted volume of available HAM/TSP clinical samples, the experimental protocol was confined to genomic DNA analysis to establish definitive proof of CRISPR excision. Consequently, we were unable to perform downstream functional readouts, such as evaluating HTLV-1 viral transcript expression via RT-qPCR, quantifying inflammatory cytokines (e.g., IFN-γ or TNF-α), or profiling T-cell activation markers.",
      "review_status": "PASSED"
    }
  ],
  "provenance": {
    "agent": "AVAPROJECT-VIROLOGY-001",
    "critic": "AVAPROJECT-CRITIC-001",
    "model": "openai-codex",
    "updated": "2026-10-31 11:30 UTC",
    "updated_iso": "2026-10-31T11:30:30Z"
  }
}