{
  "slug": "bet-inhibitor-jq1-attenuates-feline-leukemia-virus-dna-proviru-37766260",
  "source": {
    "title": "BET Inhibitor JQ1 Attenuates Feline Leukemia Virus DNA, Provirus, and Antigen Production in Domestic Cat Cell Lines.",
    "journal": "Viruses",
    "year": 2023,
    "doi": "10.3390/v15091853",
    "pmid": "37766260",
    "content_access_status": "FULL_TEXT_OA"
  },
  "claims": [
    {
      "statement": "In FeLV-exposed 3201 cells, the lower normalized proviral load observed with 500 nM (+)-JQ1 was not statistically significant.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "100",
      "evidence_excerpt": "Although not statistically significant, the fALB-normalized FeLV proviral load in 500 nM (+)-JQ1-treated, FeLV-exposed 3201 cells trended lower compared to controls at all measured timepoints ( Figure 4 a).",
      "review_status": "PASSED"
    },
    {
      "statement": "In FeLV-challenged 81C cultures, (+)-JQ1 significantly reduced feline-albumin-normalized proviral loads at 125 and 500 nM at 48, 72, and 192 h post-infection compared with enantiomer and untreated controls.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "100",
      "evidence_excerpt": "Significant dose-dependent decreases in fALB-normalized FeLV proviral loads of 81C cell cultures treated with 125 nM and 500 nM (+)-JQ1 and challenged with FeLV were observed at 48, 72, and 192 hpi compared to enantiomer and untreated controls ( Figure 2 a).",
      "review_status": "PASSED"
    },
    {
      "statement": "The authors identify testing additional cell lines as necessary because FeLV has broad cell tropism.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "100",
      "evidence_excerpt": "Due to broad FeLV cell tropism, the effects of BETi on FeLV infection in additional cell lines should be assessed.",
      "review_status": "PASSED"
    },
    {
      "statement": "In 3201 cells exposed to 500 nM (+)-JQ1, the proportion of unintegrated FeLV DNA was significantly greater than in controls at 24 h post-infection.",
      "epistemic_type": "EVIDENCE",
      "evidence_level": "D",
      "confidence": "100",
      "evidence_excerpt": "The proportion of unintegrated FeLV DNA in 3201 cells treated with 500 nM (+)-JQ1 was significantly greater at 24 hpi compared to controls ( Figure 4 b).",
      "review_status": "PASSED"
    }
  ],
  "provenance": {
    "agent": "AVAPROJECT-VIROLOGY-001",
    "critic": "AVAPROJECT-CRITIC-001",
    "model": "openai-codex",
    "updated": "2026-10-31 11:30 UTC",
    "updated_iso": "2026-10-31T11:30:30Z"
  }
}